Effects of carbon dioxide anaesthesia on survival of giant freshwater prawn Macrobrachium rosenbergii
YANG Feng1, WANG Yixin1, SHEN Siyuan1, WU Han1, SHI Wenzheng1,2, WANG Zhihe1,2,3*
1.College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China; 2.National Research and Development Center for Processing Technology of Freshwater Aquatic Products (Shanghai), Shanghai 201306, China; 3.Shanghai Aquatic Product Processing and Storage Engineering Technology Research Center, Shanghai 201306, China
Abstract: Effects of CO2 anesthesia concentrations of 60, 120, 180, 240, and 300 mg/L on survival time and survival rate of giant freshwater prawn Macrobrachiumrosenbergii with body weight of 20-30 g were investigated at prawn to water ratio in weight of 1∶3, 1∶6, 1∶9, and 1∶12 and at water temperature of 12, 16, 20, and 25 ℃. The optimization of CO2 anesthesia and the changes in water quality and physical and chemical indices were evaluated in muscle, blood, liver and other tissues of giant freshwater prawn under the conditions of CO2 concentrations of 150, 180, and 210 mg/L, at water temperature of 18, 20, and 22 ℃ and at prawn to water ratio in weight of 1∶8, 1∶9, and 1∶10 during the keep-alive process in an orthogonal test. The results showed that over 90% of survival within 48 h was observed in the giant freshwater prawn exposed to CO2 anaesthesia concentration of 150 mg/L, at 18 ℃ and at prawn to water ratio in weight of 1∶10. Under these conditions, the levels of total ammonia nitrogen, and dissolved oxygen and pH in water were within the suitable survival range, and levels of adenosine triphosphate(ATP), muscle glycogen and liver glycogen were significantly decreased to some extent(P<0.05). The activities of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) in blood were found to be increased significantly. The activity of T-SOD and MDA content in liver were negatively correlated with CO2 concentration and significantly changed. The findings indicated that CO2 anesthesia alleviated the stress of prawn body and controlled the change in water quality index, so as to reduce the metabolism of body muscles, blood and liver tissues to improve the survival time and survival rate to some extent.
2020, Vol. 35 
