Effects of dietary raffinose on intestinal morphology and microbiome, non-specific immunity, growth and ornamental value of koi carp Cyprinus carpio var.koi
LI Tieliang, XING Wei, XU Guanling, MA Zhihong, JIANG Na, YU Huanhuan, LUO Lin*
Beijing Fisheries Research Institute, Beijing 100068, China
Abstract: Koi carp Cyprinuscarpio var. koi juveniles with body weight of (42.34±0.52)g were reared in a circulating filtration aquaculture pond of 2.0 m×1.2 m ×1.5 m at stocking rate of 30 fish per pond at water temperature of (22.33±1.35)℃ and fed basic diet as control group (CK), and basic diet containing 0.06% raffinose (group T1) and 0.10% raffinose (group T2) for 70 days to investigate the effects of raffinose on intestinal morphology and microbiome, non-specific immunity, growth and ornamental value of koi carp. The results showed that there was higher density of microvilli and the gut microbial diversity in the fish in groups T1 and T2 than those in the control group. Scanning electron microscope revealed that fewer and smaller chyme particles in the intestines of koi carp in groups T1 and T2. The koi carp in groups T1 and T2 had significantly higher serum superoxide dismutase(SOD) activity than the koi carp in the control group (P<0.05) did, without significant difference in other immune or antioxidant indices among three groups. No change in the food intake rate was observed in koi carp in T1 and T2 groups, and the weight gain rate, specific growth rate and L* value in fish body surface were significantly higher in koi carp in T1 and T2 groups than those in the control group (P<0.05), and the whole fish fat content was lower than that in the control group (P<0.05). The intestine health, non-specific immunity and ornamental value such as growth, and L* value in fish body surface of koi carp in group T1 were not inferior, even superior to those in group T2. In conclusion，dietary raffinose content of 0.06% leads to improve intestinal health, non-specific immunity, the growth and ornamental value of koi carp.
发布日期: 2020-12-18
期的出版日期: 2020-12-18
中图分类号:
S963.73
基金资助: Cyprinus carpio var.koi|raffinose|intestine|non-specific immunity|growth|ornamental value
引用本文:
李铁梁, 邢薇, 徐冠玲, 马志宏, 姜娜, 郁欢欢, 罗琳. 棉子糖对锦鲤肠道健康、非特异性免疫、生长性能及观赏品质的影响[J]. 大连海洋大学学报, 2020, 35(6): 822-829.
LI Tieliang, XING Wei, XU Guanling, MA Zhihong, JIANG Na, YU Huanhuan, LUO Lin. Effects of dietary raffinose on intestinal morphology and microbiome, non-specific immunity, growth and ornamental value of koi carp Cyprinus carpio var.koi. Journal of Dalian Ocean University, 2020, 35(6): 822-829.
2020, Vol. 35 
