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大连海洋大学学报  2020, Vol. 35 Issue (3): 339-346    DOI: 10.16535/j.cnki.dlhyxb.2020-032
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日本真海带褐藻聚糖硫酸酯降血脂功效及其相关基因表达研究
王俊鑫,王亚芳,韩梓琪,何云海,任丹丹,武龙,丛海花,汪秋宽*
大连海洋大学 食品科学与工程学院,国家海藻加工技术研发分中心,辽宁水产品加工及综合利用重点实验室,辽宁 大连
Hypolipidemic effect and related gene expression of fucoidan from kelp Laminaria japonica
WANG Junxin, WANG Yafang, HAN Ziqi, HE Yunhai, REN Dandan, WU Long, CONG Haihua, WANG Qiukuan*
National Research & Development Branch Center For Seaweed Processing,Key Laboratory of Fishery Product Processing and Utilization of Liaoning Province,College of Food Science and Engineering,Dalian Ocean University, Dalian
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摘要 为研究日本真海带Laminaria japonica褐藻聚糖硫酸酯(LF)的降血脂效果及其机制,以高脂血症Wistar大鼠模型为对象,试验设置空白对照组、模型组、阳性药物组及LF低剂量(100 mg/kg·d)、LF高剂量(300 mg/kg·d)组,每日投饲一次、灌胃一次,试验共进行12周,在第4、8、12周时测定各组血清脂质指标、肝脏抗氧化酶活性及胆固醇代谢、脂质合成与分解相关基因的表达,并观察其肝脏组织变化。结果表明:日本真海带褐藻聚糖硫酸酯的总糖含量为75.27%、硫酸基含量为19.58%,LF主要由岩藻糖(41.61%)和半乳糖(24.90%)单糖组成,同时还含有葡萄糖、葡萄糖醛酸、木糖、果糖;降血脂试验中,通过与模型组相比,LF对高脂饲料引起的Wistar大鼠血清总胆固醇(TC)、甘油三酯(TG)水平上升有显著的抑制作用(P<0.05),灌胃12周后效果最佳;灌胃LF 12周后,Wistar大鼠的高密度脂蛋白(HDL-C)水平显著提升(P<0.05)、低密度脂蛋白(LDL-C)水平显著降低(P<0.05);灌胃高剂量LF 8周和12周时对大鼠肝脏MDA水平表现出显著抑制作用(P<0.05),灌胃LF后可显著提升Wistar大鼠肝脏SOD和谷胱甘肽过氧化物酶(GSH-Px)活性(P<0.05);灌胃LF组大鼠肝脏HMG-CoA表达量下调和LCAT表达量上升,表明LF降血脂作用主要通过控制胆固醇和脂肪酸合成实现的,SREBP-1c表达量下调和SCD1表达量上升,表明LF能减缓脂质合成;肝组织显微结构观察显示,喂食LF的大鼠肝脏脂肪化显著降低。研究表明,真海带褐藻聚糖硫酸脂通过抑制胆固醇和脂肪酸的合成、促进脂肪的分解从而达到降血脂作用,其降血脂效果与降血脂药物相当,可作为开发新型降血脂保健食品或药品的原料。
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王俊鑫
王亚芳
韩梓琪
何云海
任丹丹
武龙
丛海花
汪秋宽
关键词:  日本真海带  褐藻聚糖硫酸酯  单糖组成  降血脂    
Abstract: The chemical composition and monosaccharide composition of fucoidan from kelp Laminaria japonica (LF) were analyzed by phenol-sulfuric acid method, hydrochloric acid hydrolysis-barium sulfate gravimetric method, and high performance liquid chromatography. Wistar rats with body weight of (150.0±10.0)g were fed basic diet (blank control, group) and the high fat diet containing 10% of pork oil and 2% cholesterol and 0.5% pig bile salt and then orally administrated at kelp fucoidan dose of 100 mg/(kg·d) (low dose) and 300 mg/(kg·d) (high dose)for 12 weeks to evaluate the hypolipidemic activity and mechanism (including histological structure of liver and mRNA expression of related genes of rat) of kelp fucoidan. It was found that the fucoidan (LF) of the kelp was comprised of 75.27% total sugar and 19.58% sulfate group and that the monosaccharides of LF were primarily composed of fucose (41.61%) and galactose (24.90%), with glucose, glucuronic acid, xylose, and fructose. The hypolipidemic test showed that the fucoidan significantly suppressed the total cholesterol (TC) and triglyceride (TG) levels in serum of Wistar rats induced by the high-fat diet, with the best effect in 12 weeks of gastric administration. Wistar rats in the low and high dose groups had significant increase in high density lipoprotein (HDL-C) level and decrease in low density lipoprotein (LDL-C) level after 12 weeks of gavage. The significant inhibitory effects of LF on MDA levels was observed in high-dose group at 8 and 12 weeks, and the significant increase in SOD activity was found in all experimental groups. There was significant increase in GSH-Px activity in Wistar rats in high and low dose LF groups. The decrease in HMG-CoA mRNA expression level and the increase in LCAT mRNA expression level were shown in the livers of Wistar rats in LF low and high dose groups, indicating that the blood lipid-lowering effect of LF is primarily derived from regulation of the synthesis of cholesterol and fatty acids. The decrease in SREBP-1c mRNA expression and the increase in SCD1 mRNA expression further led to slow down lipid synthesis. The histological observation showed significant decrease in liver lipids in the rats fed LF, illustrating the hypolipidemic activity by intragastric administration of LF. The findings indicated that LF inhibited the synthesis of cholesterol and fatty acids, and promoted the decomposition of fat to improve the body’s hyperlipidemia, and that LF can be used as a raw material for development of new hypolipidemic health foods or medicines.
Key words:  Laminaria japonica    fucoidan    monosaccharide composition    hypolipidemia
               出版日期:  2020-05-22      发布日期:  2020-05-22      期的出版日期:  2020-05-22
中图分类号:  TS254  
基金资助: 国家重点研发计划项目(2016YFF0201802);国家海藻产业技术体系专项(CARS-50)
引用本文:    
王俊鑫, 王亚芳, 韩梓琪, 何云海, 任丹丹, 武龙, 丛海花, 汪秋宽. 日本真海带褐藻聚糖硫酸酯降血脂功效及其相关基因表达研究[J]. 大连海洋大学学报, 2020, 35(3): 339-346.
WANG Junxin, WANG Yafang, HAN Ziqi, HE Yunhai, REN Dandan, WU Long, CONG Haihua, WANG Qiukuan. Hypolipidemic effect and related gene expression of fucoidan from kelp Laminaria japonica. Journal of Dalian Ocean University, 2020, 35(3): 339-346.
链接本文:  
https://xuebao.dlou.edu.cn/CN/10.16535/j.cnki.dlhyxb.2020-032  或          https://xuebao.dlou.edu.cn/CN/Y2020/V35/I3/339
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