Abstract: Rotifer Brachionusplicatilis was cultured in a 15 L glass jar at a density of 20 ind./mL under continuous aeration and fed 7 kinds of diets including alga Nannochloropsis sp. (A), vitamin C fermentation waste (B), alga Schizochytrium sp. (C), alga Nannochloropsis sp.+ vitamin C fermentation waste (D), alga Nannochloropsis sp.+ Schizochytrium sp. (E), Nannochloropsis sp. + vitamin C fermentation waste + Schizochytrium sp. (F), and Nannochloropsis sp. mixed with solid fermentation waste (G) at water temperature of (29.0±1.4)℃, and salinity of 23.0±1.1 for 7 days to evaluate the potentiality of rotifer cultivation with vitamin C fermentation wastes. The maximal density (211 ind./mL)of the rotifer was found in the group (G), with the maximal carrying egg rate of (69.4±7.1)%, average daily production of(27.3±4.6)ind./(mL·d) and average daily growth rate of (33.5±2.6)%/d and the minimal density (11 ind./mL) of the rotifer in group B. Descending order of the rotifer density was expressed as group G>group E>group D>group F>group A>group C>group B. There were high concentrations of ammonia nitrogen, nitrite, and nitrite in group B, group D and group G, containing fermentation waste, with the maximal concentration of ammonia nitrogen (13.81 mg/L) in group B. The pH values were found to be decreased in all the groups, especially in group B (7.13) and group D (7.17) at the end of the experiment, without obvious change. In conclusion, vitamin C fermentation waste is a potential food for rotifer culture, and it is recommended that the vitamin C fermentation waste be fed as solid mass without liquid containing high concentration of ammonia nitrogen, and mixed with other food such as microalgae.